VS200
The Power to See More
Easily analyze, share, and archive your data with the SLIDEVIEW VS200 research slide scanner. Designed to capture high-resolution images of your slides for quantitative analysis, the system enables you to make the most of the information your slides have to offer.
*This product is not available in some areas
Outstanding Image Quality for Quantification
Better Resolution and Flatness
- Uses X Line high-performance objectives to deliver images with no stitching artifacts or patchwork effect.
- An optimized optical beam path provides more homogenous illumination.
- High-power True Color LED matches the spectral performance of reference halogen light sources, so brightfield stains like purple, cyan, and pink are correctly represented, imaged, and rendered.
- Fluorescence illuminator with a fly-eye lens uniformly distributes light for bright, even images.
- Optional optical sectioning device produces high-contrast images, especially for thick samples.
Flexible for Many Applications
Supports Multiple Hardware Options
The VS200 slide scanner is a highly flexible system that supports various slide sizes, different objectives, the SILA optical sectioning device, oil dispenser, cameras, loaders, and LED/laser light sources with multiple filter sets.
Supports Multiple Observation Methods
The VS200 supports brightfield, darkfield, polarization, phase contrast, and fluorescence, with the possibility to scan a sample using multiple techniques at the same time.
Flexible Software Solutions
The VS200 software supports offline analysis, deep learning, 3D deconvolution, and batch conversion to standard file formats. It also supports the NIS-SQL database for data management and the DICOM format for easy integration into a lab information management system (LIMS).
Human cartilage captured with X Line UPLXAPO10X objective.
Achieve More in Less Time
- Loader holds up to 35 sample trays with a maximum capacity of 210 26 × 76 mm (1 × 3 in.) slides
- Robotics quickly and safely loads and unloads trays while coordinating with the focus and scan unit for fast acquisition
- Integrated barcode reader automatically captures and records slide information
Simplified and Powerful Workflow
- Simple user interface—choose expert mode to customize the system’s settings or quick mode where the software optimizes the settings for you
- Save and recall settings to speed up repetitive workflows and standardize operations
- Settings can be saved as projects and shared among multiple users
- TruAI technology—post-processing deep learning enables accurate segmentation of morphological features on a sample
Reliable Data for Many Applications
The flexibility of five imaging modes and outstanding Olympus X Line objectives make the VS200 slide scanner a solution for many applications.
Brain Research
Cortico-thalamic projection pathways labeled with AAV-GFP and AAVtdTomato.
Image data courtesy of Hong Wei Dong, MD, PhD, Professor of Neurology, Keck School of Medicine of University of Southern California.
Cancer and Stem Cell Research
Tonsil CD3 (rm), ImmPRESS Reagent (HRP) Anti-Mouse IgG Immpact DAB (brown), AE1/AE3(m) ImmPRESS (AP) (HRP) Anti-Rabbit IgG Immpact Vector Red (red). Counterstained with Hematoxylin QS (blue).
Image data courtesy of Vector Labs.
Drug Discovery
Pancreas stained with Dapi, GFP and RFP.
Image data courtesy of Wenjin Chen, NJ Rutgers Cancer Center.
Testimonials
Michel Meijer
Technician, Digital and Computational Pathology Unit
University Medical Center Groningen (UMCG)
“Within the slide scanning facility of the pathology department there was a high demand for a whole slide scanning microscope with multiple methods of illumination (brightfield, polarization, and fluorescence) and a diverse range of objectives (air and oil). The SLIDEVIEW VS200 scanner fits our needs as a day-to-day whole slide scanning system for research purposes without compromising on image quality.”
Applied Technologies
High-Resolution Scanning
High optical resolution and image quality enable visual or automated analysis of image information
Immersion available in manual and automatic modes
Automatic immersion unit for high-resolution oil (40X, 60X, and 100X) or silicone oil (40X) immersion objectives
Applied Technologies
Tonsil CD3 (rm), ImmPRESS Reagent (HRP) Anti-Mouse IgG Immpact DAB (brown), AE1/AE3(m) ImmPRESS (AP) (HRP) Anti-Rabbit IgG Immpact Vector Red (red). Counterstained with Hematoxylin QS (blue).
Image data courtesy of Vector Labs.
Accurate Focus at All Magnifications
- Z-focus is 4X more precise than our previous slide scanner and offers higher readjustment accuracy with linear encoders
- Automatic sample detection helps determine the scan area for faster imaging while the autofocus improves the automated stitching function
- Design minimizes vibrations for better imaging stability
- Manually edit the sample mask and modify the automatically detected sample mask
Bright LED with Accurate Color Reproduction
The system’s true color LED for transmitted illumination has the same spectral characteristics and power as a halogen lamp, so purple, cyan, and pink stains are correctly represented, imaged, and rendered.
Uniform Fluorescence Illumination
The fluorescence illuminator with its fly-eye lens uniformly distributes light across the entire field of view for bright, evenly illuminated images.
High-Contrast Images without Blur
The SILA optical sectioning device quickly delivers blur-free scans of thick samples, so you can achieve widefield and section imaging with a single system.
Based on Hi-Lo microscopy, the SILA (speckle illumination acquisition) optical sectioning device produces high-contrast images free from out of focus blur. Adding the SILA hardware to a VS200 makes it especially well suited for imaging thicker samples that either normal or TruSight imaging cannot resolve effectively. The SILA device delivers real-time, blur-free scans of thick samples, so you can achieve widefield and section imaging with a single system.
Learn more about the SILA optical sectioning device
Widefield (left), Widefield + TruSight Deblurring (middle) and SILA (right) images of a whole planarian flatworm Schmidtea mediterranea in 20x, showing the intestines. Blue: DAPI. Green: inner intestine cells; Red: outer intestine cells. Samples provided by Amrutha Palavalli, Department for Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Goettingen, Germany.
Online Fluorescence Deblurring
TruSight Live deblurring reduces diffused light from above and below the focal plane of a thick sample. The image data is then recalculated using a special 2D deconvolution algorithm, making the images sharper and clearer.
Learn more about the applicationOnline Fluorescence Deblurring
Widefield (left), Widefield + TruSight Deblurring (middle) and SILA (right) images of a whole planarian flatworm Schmidtea mediterranea in 20x, showing the intestines. Blue: DAPI. Green: inner intestine cells; Red: outer intestine cells. Samples provided by Amrutha Palavalli, Department for Tissue Dynamics and Regeneration, Max Planck Institute for Multidisciplinary Sciences, Goettingen, Germany.
Lung tissue imaged on a VS200 at 20X stained with an Ultivue PD-L1 kit multiplex kit; Dapi: Nuclear Counterstain, FITC: CD8, TRITC: CD68, Cy5: PD-L1, Cy7: panCK.
Image data courtesy of Ultivue Inc.
Multiplex Scan Mode
When tissue samples are limited, it’s important to get the most possible data from each tissue section. Multiplexing mode aligns multiple fluorescence channels with a reference image, enabling greater understanding of co-expression and spatial composition of multiple targets within a sample.
The Power of TruAI Deep-Learning Technology
- Deep learning enables faint samples or morphological features on a sample to be accurately identified and scanned, saving time and effort
- Target regions, such as pancreas islets, can be accurately segmented and differentiated from erythrocytes
Left: Cy3 fluorescence marked pancreatic islets. Pancreatic islets are stained (red) while erythrocytes are autofluorescencing.
Right: Probability map detection based on TruAI technology. Only pancreatic islets are accurately detected (green).
Image data courtesy of Univ.-Prof. Dr. rer. nat. Simone E. Baltrusch, Institute for Medical Biochemistry and Molecular Biology, Rostock University Medical Center, University of Rostock.
Learn more about the application: Segmentation and Analysis of Pancreatic Islets
Learn more about TruAI technology
Advanced Automation for More Efficient Image Acquisition
Optimized Slide Scanning Workflow
- More efficient and flexible: the software is easy to set up and operate. You can upload your trained neural network and open it with the click of a button.
- Reduce the volume of acquired data: TruAI technology and selective scanning prevents the system from acquiring large amounts of data from areas you don’t care about. This helps optimize data management, including storage, uploading, and image sharing.
- Set it and forget it: TruAI technology is accurate, reducing the need for you to supervise the scanning. This enables you to use your time for other tasks or do scanning runs overnight or over the weekend.
Higher Productivity
Multibatch Processing
- Identical Settings mode automatically assigns scan settings to all the slides
- Individual Settings mode enables you to change specific settings for each slide or all the slides in a single tray
- Flexible Batch Scan mode enables you to designates different observation method such as FL, BF, POL, DF, and PH for each slide contained in the batch
- Priority Scan function enables you to interrupt a continuous operation to scan a slide and then resume what you were scanning
Hot-Swap
Hot-swap functionality enables you to exchange an already scanned slide tray with a new tray during batch processing so that you can add new slides before the system has finished scanning. This function minimizes the amount of time the system is idle.
Supports Glass Slides and Plates
- Simple-to-use slide tray enables you to mix 26 × 76 mm (1 × 3 in.), 52 × 76 mm (2 × 3 in.), 76 × 102 mm (3 × 4 in.), and 102 × 127 mm (4 × 5 in.) slides on the same batch scan
- A RFID system enables the unit to automatically detect trays, the number of slides, and the size of the slides
Data Management
Comprehensive Image and Data Management
- Conveniently manage your images with the optional Net Image Server (NIS) SQL database.
- Store and send images using the web to share your virtual slides with a broad audience.
- Control access to your data with individual access rights.
- Scan images directly to .vsi, OME-TIFF, and DICOM formats or export batches of already acquired images to many different file formats.
- Scan your images with the ability to query the LIS (laboratory information system) for additional metadata and save them in the DICOM format. You can then upload them to an IMS.
- Automatic upload to the optional Net Image Server (NIS) SQL database.
The VSI file format supported by VS200 systems was developed by EVIDENT to meet the specialized requirements of advanced microscopy imaging. A number of projects and companies are already supporting the VSI format in their applications, and more are on the way.
91360 Med Tech Co Ltd
Cirdan Imaging Limited
ImageJ
Lemke Software GmbH
netTrek
Smart Imaging Technology
Visiopharm
Learn more about the Net Image Server (NIS)Aiforia / Fimmic Oy
Corista LLC
Imaris
Media Cybernetics Inc
Pathcore Inc.
Smart in Media GmbH
Andor Technology Ltd.
Hiroya Corporation
Indica Labs
Microvolution LLC
Pathomation BV
TissueGnostics GmbH
*Alphabetical order
Remote Access with Free OlyVIA Virtual Slide Viewer
- View virtual slides on Windows PCs
- Images can be stored and opened locally or from a network
- Images saved on the NIS can be viewed over an internet connection
Barcode Reading
- The system’s barcode reader scans a large selection of 1D and 2D barcode types
- The information recorded can be configured as a file name or folder name, making it convenient to handle large number of slides
Specifications
| VS200 Single Tray | VS200 Multiple Tray Loader | ||
| Intended Specimen | Observable Specimen | Glass slide with cover glass | |
| Size of Glass Slide (W × L × H) |
Standard slide tray: 25 mm–26.5 mm × 75 mm–76.5 mm × 0.9 mm–1.2 mm (1 in. × 3 in. × 0.05 in.) (6 slides) Optional trays 1) 51 mm–53 mm × 75 mm–76.5 mm × 0.9 mm–1.2 mm (2 in. × 3 in. × 0.05 in.) (3 slides) 2) 100 mm–102 mm × 75 mm–76.5 mm × 0.9 mm–1.2 mm (4 in. × 3 in. × 0.05 in.) (1 slide) 3) 126 mm–128 mm × 75 mm–76.5 mm × 1.1 mm–1.4 mm (5 in. × 3 in. × 0.06 in.) (1 slide) |
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| Cover Glass Thickness | 0.12–0.17 mm | ||
| Observation Methods | Brightfield, reflected brightfield (optional*1), darkfield, phase contrast (optional*2), simple polarization (optional*3), fluorescence (optional), fluorescence optical sectioning with speckle illumination (optional SILA module) | ||
| Optical Frame | Illuminator | Built-in Köhler illumination for transmitted light; high intensity and high color rendering LED (up to 50,000 hours) | |
| Objectives | Compatible objectives 2x, 4x*4, 10x*5, 20x, 40x*5, 60x*5, and 100x*5 6-position motorized nosepiece (incl. selected oil immersion, silicon oil immersion, and phase contrast objectives) Optional automatic oil dispenser |
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| Motorized Stage | XY stage with automatic control | ||
| Focusing | Motorized focusing with automatic control | ||
| Color Camera | Integrated 2/3 inch CMOS, 3.45 μm × 3.45 μm pixel size, high sensitivity, high resolution | ||
| Scan Unit | Capacity | 1 slide tray, 6 slides maximum; upgradable to a multiple tray loader model | Up to 35 slide trays, 210 slides maximum |
| Pixel Resolution (Color Camera) |
UPLXAPO20X (NA 0.8): 0.274 μm/pixel Options: UPLXAPO4X (NA 0.16): 1.37 μm/pixel UPLXAPO10X (NA 0.4): 0.548 μm/pixel UPLXAPO40X (NA 0.95): 0.137 μm/pixel UPLXAPO40XO (NA 1.4): 0.137 μm/pixel UPLXAPO60XO (NA 1.42): 0.091 μm/pixel UPLXAPO100XO (NA 1.45): 0.055 μm/pixel |
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| Scan Time | Brightfield: approx. 1.5 minutes (20x objective, scan area 15 mm × 15 mm) Fluorescence widefield NOVEM: approx. 6.5 minutes (20x objective, scan area 15 mm × 15 mm, 4 fluorescence channels, 10 ms exposure each) |
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| Software | Automatic sample detection (generic and TruAI deep learning), automatic barcode reading, automatic focus mapping, automatic scanning, automatic stitching, pause and resume scanning, Z-stack imaging, extended focus imaging (EFI), image format: vsi, JPEG, TIFF, DICOM, synchronized multi-image display, stepless zooming, zooming while scanning, annotations, screen capture, slide loader control (multiple tray loader only) | ||
| Fluorescence (optional) |
Fluorescence Components | UPLFLN4X objective, illuminator with fly-eye lens, motorized mirror turret, motorized filter wheel Widefield light source options: U-LGPS, Excelitas X-Cite XYLIS, X-Cite TURBO, X-Cite Novem SILA: 4-line laser combiner (405 nm, 488 nm, 561 nm, and 638 nm) and scrambler unit |
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| Monochrome Camera | Options: VS-304M, 1-inch CMOS, 3.45 μm × 3.45 μm pixel size HAMAMATSU ORCA Flash4.0 V3 HAMAMATSU ORCA Fusion HAMAMATSU ORCA Fusion BT |
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| Solutions for Scanner Software (optional) |
Solution License | Batch image format converter DICOM converter Fluorescence SILA acquisition |
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| Desktop Software (optional, separate solution for analysis) |
Solution License | Batch image format converter DICOM converter Detection and analysis Deep learning 3D deconvolution |
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| Environment | Weight | Optical frame: 69 kg (152.1 lb) 1 slide tray: 0.6 kg (1.3 lb) |
Optical frame and multiple tray loader: 142 kg (313 lb) 35 slide trays: 21 kg (46.3 lb) |
| Fluorescence: 8 kg (17.6 lb) PC and monitor: 16 kg (35.3 lb) Camera cover (optional): 9 kg (19.8 lb) |
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| Operating Environment | Temperature: 15–28 °C (59–82.4 °F) (including other devices) Humidity: up to 80% (31 °C (87.8 °F)) |
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| Power Supply*5 | Input: AC 100–240 V, 50/60 Hz, 4 A Output: DC 24 V, 9.2 A |
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| Power Consumption | Input: 100–240 V AC; 50/60 Hz; 4 A 221 W |
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*1 Optional phase contrast objectives are required.
*2 Optional analyzer mirror unit and motorized fluorescence mirror turret are required.
*3 Optional analyzer mirror unit and motorized mirror turret are required
*4 Included in Fluorescence Solution
*5 Sold separately
Resources
Application Notes
Identifying Cirrhosis in Liver Tissue Using a Research Slide Scanner
Rapid Automated Detection and Segmentation of Glomeruli Using Self-Learning AI Technology
Digitizing Very Thick Fluorescent Samples with the SLIDEVIEW VS200 Research Slide Scanner
Application Notes
Whole Slide Imaging with Oil Immersion Media: Frequently Asked Questions (FAQs)
5 Practical Ways to Accelerate Your Microscopy Experiments
From Multimode Imaging to Multiplexing: Your Essential Guide to Extracting Rich Data